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ERX5504060: NextSeq 500 sequencing; Deciphering the regulatory role of CsrA in Leptospira biflexa
1 ILLUMINA (NextSeq 500) run: 19M spots, 2.6G bases, 1.2Gb downloads

Design: Deciphering the regulatory role of CsrA in Leptospira biflexa
Submitted by: 1. Plate-forme Technologique Biomics ??“ Centre de Ressources et Recherches Technologiques (C2RT), Institut Pasteur, Paris, France 2. Hub de Bioinformatique et Biostatistique - Departement Biologie Computationnelle, Institut Pasteur, 75015 Paris, France (1. Plate-forme Technologique Biomics ??“ Centre de)
Study: Deciphering the regulatory role of CsrA in Leptospira biflexa
show Abstracthide Abstract
Characterization of the role of CsrA in gene regulation in Leptospira biflexa
Sample: comp_csrA1
SAMEA8657178 • ERS6341726 • All experiments • All runs
Library:
Name: comp_csrA1_s
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Inverse rRNA
Layout: SINGLE
Construction protocol: centrifugation of exponential-phase cultures in EMJH medium After treatment of cells with Trizol, ucleic Acids were extracted with chloroform and precipitated with isopropanol The QIAseq Fast Select -5S/16S/23S (QIAGEN) kit was used for ribosomal RNA depletion according to manufacturer instructions. Libraries were built using a TruSeq Stranded mRNA library Preparation Kit (Illumina, USA) following the Qiaseq Fastselect -5S/16S/23S protocol recommendations. Quality control was performed on a Fragment Analyzer
Experiment attributes:
Experimental Factor: genotype: complemented mutated csrA
Runs: 1 run, 19M spots, 2.6G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
ERR585609118,995,4972.6G1.2Gb2022-04-13

ID:
21271513

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